Antibody was affinity purified using an epitope specific to BTF immobilized on solid support. The epitope recognized by A300-609A maps to a region between residues 300 and 350 of human BCL2-Associated Transcription Factor 1 using the numbering given in entry NP_055554.1 (GeneID 9774). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bcl-2-associated transcription factor 1, BCLAF1 and THRAP3 family member 1, bK211L9.1, BTF
between 300 and 350
2 - 8°C
Detection of human BTF by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BTF antibody BL2522 (Cat. No. A300-609A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BTF was also immunoprecipitated using rabbit anti-BTF antibodies BL2521 (Cat. No. A300-608A) and BL2523 (Cat. No. A300-610A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
Detection of human BTF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BTF (Cat. No. A300-609A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB