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Rabbit anti-CAP-G2 Antibody, Affinity Purified - A300-605A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CAP-G2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-605A
Name:
Rabbit anti-CAP-G2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CAP-G2 immobilized on solid support.The epitope recognized by A300-605A maps to a region between residue 1100 and the C-terminus (residue 1143) of human Chromosome-Associated Protein G2 using the numbering given in entry AAH43404.1 (GeneID 54892).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
CAPG2, CAP-G2, chromosome-associated protein G2, condensin-2 complex subunit G2, hCAP-G2, leucine zipper protein 5, LUZP5, more than blood homolog, MTB, Non-SMC condensin II complex subunit G2
Species Reactivity:
Human
Immunogen:
Between 1100 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human CAP-G2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody A300-605A (lot A300-605A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody A300-605A (lot A300-605A-3) used for IP at 6 µg per reaction. CAP-G2 was also immunoprecipitated by rabbit anti-CAP-G2 antibody BL2946. For blotting immunoprecipitated CAP-G2, A300-605A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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