Antibody was affinity purified using an epitope specific to CAP-H immobilized on solid support. The epitope recognized by A300-603A maps to a region between residue 700 and the C-terminus (residue 741) of human Chromosome Associated Protein H (Barren homolog BRRN1) using the numbering given in entry NP_056156.2 (GeneID 23397). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
barren homolog 1, Barren homolog protein 1, BRRN1, CAPH, CAP-H, chromosome-associated protein H, condensin complex subunit 2, hCAP-H, MCPH23, Non-SMC condensin I complex subunit H, XCAP-H homolog
between 700 and C-term
2 - 8°C
Detection of human CAP-H by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-CAP-H antibody A300-603A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate (B). CAP-H was also immunoprecipitated using rabbit anti-CAP-H antibody BL2939, which binds an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).