Antibody was affinity purified using an epitope specific to CAP-G immobilized on solid support. The epitope recognized by A300-602A maps to a region between residue 950 and the C-terminus (residue 1015) of human Chromosome Associated Protein G (Chromosome Condensation Protein G) using the numbering given in entry NP_071741.2 (GeneID 64151). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPG, CHCG, chromosome condensation protein G, chromosome-associated protein G, condensin complex subunit 3, condensin subunit CAP-G, hCAP-G, melanoma antigen NY-MEL-3, Non-SMC condensin I complex subunit G, NY-MEL-3, XCAP-G homolog, YCG1
between 950 and C-term
2 - 8°C
Detection of human CAP-G by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-CAP-G antibody A300-602A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate (B). CAP-G was also immunoprecipitated using rabbit anti-CAP-G antibody BL2935, which binds an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).