Antibody was affinity purified using an epitope specific to CAP-D2 immobilized on solid support. The epitope recognized by A300-601A maps to a region between residue 1350 and the C-terminus (residue 1401) of human Chromosome Associated Protein D2 (Chromosome Condensation-Related SMC-Associated Protein 1) using the numbering given in entry NP_055680.2 (GeneID 9918). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAP-D2, chromosome condensation-related SMC-associated protein 1, chromosome-associated protein D2, CNAP1, condensin complex subunit 1, hCAP-D2, MCPH21, Non-SMC condensin I complex subunit D2, XCAP-D2 homolog
between 1350 and C-term
2 - 8°C
Detection of human CAP-D2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-CAP-D2 antibody A300-601A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate (B). CAP-D2 was also immunoprecipitated using rabbit anti-CAP-D2 antibody BL2931, which binds an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).