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Rabbit anti-GNL3 Antibody, Affinity Purified - A300-599A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-GNL3 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-599A
Name:
Rabbit anti-GNL3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to GNL3 immobilized on solid support. The epitope recognized by A300-599A maps to a region between residues 1 and 50 of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
C77032, E2IG3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), guanine nucleotide-binding protein-like 3, NNP47, novel nucleolar protein 47, NS, nucleolar GTP-binding protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse GNL3 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 µg) and mouse 3T3 (M, 50 µg) cells. Antibodies: Affinity purified rabbit anti-GNL3 antibody BL2856 (Cat. No. A300-599A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies BL2855 and BL2858 (Cat. No. A300-600A). Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

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Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (Cat. No. A300-599A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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