Antibody was affinity purified using an epitope specific to GNL3 immobilized on solid support. The epitope recognized by A300-599A maps to a region between residues 1 and 50 of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C77032, E2IG3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), guanine nucleotide-binding protein-like 3, NNP47, novel nucleolar protein 47, NS, nucleolar GTP-binding protein
between 1 and 50
2 - 8°C
Detection of human and mouse GNL3 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T, 50 µg) and mouse 3T3 (M, 50 µg) cells. Antibodies: Affinity purified rabbit anti-GNL3 antibody BL2856 (Cat. No. A300-599A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies BL2855 and BL2858 (Cat. No. A300-600A). Detection: Chemiluminescence with exposure times of 10 seconds (A and B).