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Rabbit anti-AF9 Antibody, Affinity Purified

Catalogue number:
A300-597A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to AF9 immobilized on solid support. The epitope recognized by A300-597A maps to a region between residue 525 and the C-terminus (residue 568) of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) using the numbering given in entry NP_004520.1 (GeneID 4300). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human AF9 by western blot a
Product Type:

Antibodies

Alternative Names:

AF9, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3, myeloid/lymphoid or mixed-l

Species Reactivity:

Human

Immunogen:

between 525 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human AF9 by western blot a

Detection of human AF9 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 10 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF9 antibody BL2313 (Cat. No. A300-597A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies BL2310 (Cat. No. A300-595A), BL2311 (Cat. No. A300-596A) and BL2312 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).