Rabbit anti-AF9 Antibody, Affinity Purified - A300-596A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to AF9 immobilized on solid support. The epitope recognized by A300-596A maps to a region between residues 250 and 300 of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) using the numbering given in entry NP_004520.1 (GeneID 4300). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human AF9 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 10 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF9 antibody BL2311 (Cat. No. A300-596A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies BL2310 (Cat. No. A300-595A), BL2312 and BL2313 (Cat. No. A300-597A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human AF9 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 (Cat. No. A300-596A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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