Antibody was affinity purified using an epitope specific to AF9 immobilized on solid support. The epitope recognized by A300-596A maps to a region between residues 250 and 300 of human ALL1 Fused Gene from Chromosome 9 Protein (MLLT3, Myeloid/Lymphoid or Mixed-Lineage Leukemia Translocate 3) using the numbering given in entry NP_004520.1 (GeneID 4300). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AF9, ALL1-fused gene from chromosome 9 protein, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3, myeloid/lymphoid or mixed-l
between 250 and 300
2 - 8°C
Detection of human AF9 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 10 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF9 antibody BL2311 (Cat. No. A300-596A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies BL2310 (Cat. No. A300-595A), BL2312 and BL2313 (Cat. No. A300-597A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).