Antibody was affinity purified using an epitope specific to Sec23IP/MSTP053) immobilized on solid support. The epitope recognized by A300-594A maps to a region between residue 950 and the C-terminus (residue 1000) of human Sec23-Interacting Protein using the numbering given in entry NP_009121.1 (GeneID 11196). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MSTP053, P125, P125A, SEC23-interacting protein
between 950 and C-term
2 - 8°C
Detection of human Sec23IP(MSTP053) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Sec23IP(MSTP053) antibody BL2566 (Cat. No. A300-594A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate. Sec23IP(MSTP053) was also immunoprecipitated using rabbit anti-Sec23IP(MSTP053) antibodies BL2564 (Cat. No. A300-592A) and BL2565 (Cat. No. A300-593A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
Detection of human Sec23IP(MSTP053) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 Sec23IP(MSTP053) (Cat. No. A300-594A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB