Antibody was affinity purified using an epitope specific to Sec23IP/MSTP053 immobilized on solid support. The epitope recognized by A300-592A maps to a region between residues 1 and 50 of human Sec23-Interacting Protein using the numbering given in entry NP_009121.1 (GeneID 11196). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MSTP053, P125, P125A, SEC23-interacting protein
between 1 and 50
2 - 8°C
Detection of human Sec23IP(MSTP053) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (50 µg for WB) cells. Antibody: Affinity purified rabbit anti-Sec23IP(MSTP053) antibody BL2564 (Cat. No. A300-592A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).