Antibody was affinity purified using an epitope specific to Matrin 3 immobilized on solid support. The epitope recognized by A300-591A maps to a region between residue 800 and the C-terminus (residue 847) of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ALS21, matrin-3, MPD2, VCPDM, vocal cord and pharyngeal weakness with distal myopathy
between 800 and C-term
2 - 8°C
Detection of human and mouse Matrin 3 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP loaded), HEK293T (T, 50 µg for WB) and mouse NIH 3T3 (M, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody BL2526 (Cat. No. A300-591A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used in IP at 3 µg/mg lysate (B). Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 antibodies BL2524 and BL2525 (Cat. No. A300-590A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human and mouse Matrin 3 by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB
Detection of human expndtw-6 Matrin 3 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:400 (0.5µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.