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Rabbit anti-Matrin 3 Antibody, Affinity Purified - A300-591A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Matrin 3 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-591A
Name:
Rabbit anti-Matrin 3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Matrin 3 immobilized on solid support. The epitope recognized by A300-591A maps to a region between residue 800 and the C-terminus (residue 847) of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, IHC-IF

Product Type:
Antibodies
Alternative Names:
ALS21, matrin-3, MPD2, VCPDM, vocal cord and pharyngeal weakness with distal myopathy
Species Reactivity:
WB, IP, IHC, IHC-IF
Immunogen:
Between 800 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
WB, IP, IHC, IHC-IF
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Matrin 3 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP loaded), HEK293T (T, 50 µg for WB) and mouse NIH 3T3 (M, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody BL2526 (Cat. No. A300-591A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used in IP at 3 µg/mg lysate (B). Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 antibodies BL2524 and BL2525 (Cat. No. A300-590A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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Detection of human and mouse Matrin 3 by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB

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Detection of human expndtw-6 Matrin 3 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:400 (0.5µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

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