Antibody was affinity purified using an epitope specific to Matrin 3 immobilized on solid support. The epitope recognized by A300-590A maps to a region between residues 475 and 500 of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ALS21, matrin-3, MPD2, VCPDM, vocal cord and pharyngeal weakness with distal myopathy
between 475 and 500
2 - 8°C
Detection of human Matrin 3 by western blot after immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody BL2525 (Cat. No. A300-590A) used for IP at 3 µg/mg lysate. Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 antibodies BL2524 and BL2526 (Cat. No. A300-591A) at 3 µg/mg lysate. immunoprecipitatesd Matrin 3 was blotted using BL2526 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human and mouse Matrin 3 by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 (Cat. No. A300-590A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB