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Rabbit anti-VCP Antibody, Affinity Purified - A300-589A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-VCP Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-589A
Name:
Rabbit anti-VCP Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to VCP immobilized on solid support.The epitope recognized by A300-589A maps to a region between residue 750 and the C-terminus (residue 806) of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
15S Mg(2+)-ATPase p97 subunit, CDC48, p97, TER ATPase, TERA, transitional endoplasmic reticulum ATPase, Valosin-containing protein, VCP
Species Reactivity:
Human, Mouse
Immunogen:
Between 750 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody A300-589A (lot A300-589A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody A300-589A (lot A300-589A-3) used for IP at 3 µg per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody A300-588A. For blotting immunoprecipitated VCP, A300-589A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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