Antibody was affinity purified using an epitope specific to NONO immobilized on solid support. The epitope recognized by A300-587A maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, MRXS34, NMT55, NonO protein, non-POU domain-containing octamer (ATGCAAAT) binding protein, non-POU domain-containing octamer-binding protein
Human, Mouse, Rat
between 350 and 400
2 - 8°C
Detection of human NONO by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NONO antibody BL2553 (Cat. No. A300-587A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NONO was also immunoprecipitated using anti-NONO antibody BL2252 (Cat. No. A300-582A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of mouse and rat NONO by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-NONO antibody A300-587A (lot A300-587A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human and mouse NONO by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO (Cat. No. A300-587A Lot1) used at a dilution of 1:1,000 (0.2 µg/ml) and 1:200 (1µg/ml). Detection: DAB