Antibody was affinity purified using an epitope specific to PRKRIR immobilized on solid support. The epitope recognized by A300-586A maps to a region between residues 150 and 200 of human Protein-Kinase, Interferon-inducible double strand RNA dependent Inhibitor, Repressor of (P58 Repressor) using the numbering given in entry NP_004696.2 (GeneID 5612). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
52 kDa repressor of the inhibitor of the protein kinase, 58 kDa interferon-induced protein kinase-interacting protein, DAP4, death-associated protein 4, inhibitor of protein kinase PKR, P52rIPK, p58IPK-interacting protein, P58IPK-regulatory protein, PRKRI
between 150 and 200
2 - 8°C
Detection of PRKRIR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP; 1/4 of IP reaction loaded) and mouse NIH 3T3 (M; 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-PRKRIR antibody A300-586A (lot A300-586A-1) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). PRKRIR was also immunoprecipitated using rabbit anti-PRKRIR antibody BL2254 at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human and mouse PRKRIR by immunohistochemistry. Sample: FFPE sections of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PRKRIR (Cat. No. A300-586A Lot1) used at a dilution of 1:1,000 (0.2µg/ml) and 1:200 (1µg/ml). Detection: DAB