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Rabbit anti-Phospho RPS6 (S235/236) Antibody, Affinity Purified - A300-584A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Phospho RPS6 (S235/236) Antibody, Affinity Purified
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Size: 20 µg
£322.00
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Catalogue number:
A300-584A
Name:
Rabbit anti-Phospho RPS6 (S235/236) Antibody, Affinity Purified
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Antibody was affinity purified using a synthetic peptide representing phosphorylation at serine 235 and serine 236 and surrounding residues of RPS6 immobilized on solid support. Immunogen for A300-584A was a phosphorylated synthetic peptide, which represented a portion of human Ribosomal Protein S6 (GeneID 6194) around serine 235 and serine 236 according to the numbering given in entry NP_001001.2.Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB

Product Type:
Antibodies
Alternative Names:
40S ribosomal protein S6, phosphoprotein NP33, S6, small ribosomal subunit protein eS6
Species Reactivity:
Human
Immunogen:
Surrounding serine 235/236
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Phospho RPS6 (S235/S236) by western blot. Samples: Whole cell lysate (35 µg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody (Cat. No. A300-584A) used at 0.04 µg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody (Cat. No. A300-557A) at 0.04 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).

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