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Rabbit anti-Phospho RPS6 (S235/236) Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
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£464.00 Estimated delivery Wed 3 Apr
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Applications:

WB

Antibody Host:

Rabbit

Target:

RPS6, Phospho (S235/S236)

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 235/236

Alternative Names:

S6; phosphoprotein NP33; 40S ribosomal protein S6; small ribosomal subunit protein eS6

Product Description:

Rabbit anti-Phospho RPS6 (S235/236) Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human Phospho RPS6 (S235/S2
Detection of human Phospho RPS6 (S235/S236) by western blot. Samples: Whole cell lysate (35 µg) from HeLa cells. Treatments consisted of growth in serum containing media (lane 1) or cultured for 24 hours in serum free media (lanes 2 to 5). At 24h, serum was replaced for 1 h (lanes 2 and 3) or serum starvation was continued (lanes 4 and 5) without rapamycin (lanes 3 and 5) with 50nM Rapamycin (lanes 2 and 4). Antibodies: Affinity purified rabbit anti-phospho RPS6 (S235/S236) antibody (Cat. No. A300-584A) used at 0.04 µg/ml (upper panel). The blot was stripped and re-blotted for total RPS6 using affinity purified rabbit anti-RPS6 antibody (Cat. No. A300-557A) at 0.04 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).
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