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Rabbit anti-WASP Antibody, Affinity Purified - A300-581A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-WASP Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-581A
Name:
Rabbit anti-WASP Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to WASP immobilized on solid support. The epitope recognized by A300-581A maps to a region between residue 400 and the C-terminus (residue 462) of human Wiskott-Aldrich Syndrome Protein using the numbering given in entry NP_000368.1 (GeneID 7454).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
eczema-thrombocytopenia, IMD2, SCNX, THC, THC1, thrombocytopenia 1 (X-linked), WASP, WASPA, wiskott-Aldrich syndrome protein
Species Reactivity:
Human
Immunogen:
Between 400 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human WASP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP reaction loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WASP antibody BL2485 (Cat. No. A300-581A) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used in IP at 3 µg/mg lysate. WASP was also immunoprecipitated using rabbit anti-WASP antibodies BL2483 (Cat. No. A300-580A) and BL2484. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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