Antibody was affinity purified using an epitope specific to NF2 immobilized on solid support. The epitope recognized by A300-578A maps to a region between residue 550 and the C-terminus (residue 595) of human Neurofibromin 2 using the numbering given in entry NP_000259.1 (GeneID 4771). This epitope is common to NF2 isoforms 1 and 9 (Mer162). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ACN, BANF, merlin, moesin-ezrin-radixin like, moesin-ezrin-radixin-like protein, moesin-ezrin-radizin-like protein, neurofibromin 2 (bilateral acoustic neuroma), Neurofibromin-2, SCH, schwannomerlin, schwannomin
between 550 and C-term
2 - 8°C
Detection of Isoform 1 of human NF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15, and 15 µg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and NIH293 (50 µg for WB) cells. Antibody: Affinity purified rabbit anti-NF2 antibody BL2776 (Cat. No. A300-578A) used for western blot at 0.1 µg/ml (A) and 1 µg/ml (B) for IP at 3 µg/mg lysate for IP (B). Isoform 1 of NF2 was also immunoprecipitated using rabbit ant-NF2 antibody BL2775, which is to an upstream epitope also found in isoform 2. Isoform 1 was not immunoprecipitated using rabbit anti-NF2 isoform 2 antibody BL2777 (Cat. No. A300-579A). Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).