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Rabbit anti-INPPL1/SHIP2 Antibody, Affinity Purified

Catalogue number:
A300-574A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to INPPL1/SHIP2 immobilized on solid support. The epitope recognized by A300-574A maps to a region between residues 100 and 150 of human inositol polyphosphate phosphatase-like 1 using the numbering given in entry NP_001558.2 (GeneID 3636). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

51C protein, Inositol polyphosphate phosphatase-like protein 1, INPPL-1, OPSMD, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2, protein 51C, SH2 domain-containing inositol 5'-phosphatase 2, SH2 domain-containing inositol phosphatase 2, SH2 domai

Species Reactivity:

Human, Mouse

Immunogen:

between 100 and 150

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse INPPL2/SHIP

Detection of human and mouse INPPL2/SHIP2 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa and (50 µg) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody A300-574A (lot A300-574A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human INPPL2/SHIP2 by weste

Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody A300-574A (lot A300-574A-2) used for IP at 6 µg per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (A300-574A-1) and rabbit anti-INPPL2/SHIP2 antibody BL2376. For blotting immunoprecipitated INPPL2/SHIP2, A300-574A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human expndtw-6 INPPL1/SHIP

Detection of human expndtw-6 INPPL1/SHIP2 by immunohistochemistry. Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. A300-574A Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB