Antibody was affinity purified using an epitope specific to EDD1 immobilized on solid support. The epitope recognized by A300-573A maps to a region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT domain containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DD5, E3 identified by differential display, E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EDD, EDD1, HECT-type E3 ubiquitin transferase UBR5, hHYD, HYD, hyperplastic discs protein homolog, progestin-induced prote
between 600 and 650
2 - 8°C
Detection of human and mouse EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for IP at 3 µg per reaction. EDD1 was also immunoprecipitated by rabbit anti-EDD1 antibody A303-045A. For blotting immunoprecipitated EDD1, A300-573A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.