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Rabbit anti-EDD1 Antibody, Affinity Purified - A300-573A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-EDD1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-573A
Name:
Rabbit anti-EDD1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to EDD1 immobilized on solid support. The epitope recognized by A300-573A maps to a region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT domain containing, 1 using the numbering given in entry NP_056986.2 (GeneID 51366).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
DD5, E3 identified by differential display, E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EDD, EDD1, HECT-type E3 ubiquitin transferase UBR5, hHYD, HYD, hyperplastic discs protein homolog, progestin-induced prote
Species Reactivity:
Human, Mouse
Immunogen:
Between 600 and 650
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human EDD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EDD1 antibody A300-573A (lot A300-573A-5) used for IP at 3 µg per reaction. EDD1 was also immunoprecipitated by rabbit anti-EDD1 antibody A303-045A. For blotting immunoprecipitated EDD1, A300-573A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human EDD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 5) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse EDD1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-EDD1 (Cat. No. A300-573A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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