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Rabbit anti-BLM Antibody, Affinity Purified - A300-572A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BLM Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-572A
Name:
Rabbit anti-BLM Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Blm immobilized on solid support. The epitope recognized by A300-572A maps to a region between residue 1375 and the C-terminus (residue 1416) of mouse Bloom Syndrome Homolog using the numbering given in entry NP_031576.2 (GeneID 12144).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Bloom syndrome homolog, Bloom syndrome protein homolog, Bloom syndrome, RecQ helicase-like, mBLM, recQ helicase homolog
Species Reactivity:
Human, Mouse
Immunogen:
Between 1375 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse BLM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody A300-572A (lot A300-572A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody A300-572A (lot A300-572A-2) used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot A300-572A-1) and rabbit anti-BLM antibody A300-570A For blotting immunoprecipitated BLM, A300-572A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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