Antibody was affinity purified using an epitope specific to Blm immobilized on solid support. The epitope recognized by A300-570A maps to a region between residues 1 and 50 of mouse Bloom Syndrome Homolog using the numbering given in entry NP_031576.2 (GeneID 12144). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Bloom syndrome homolog, Bloom syndrome protein homolog, Bloom syndrome, RecQ helicase-like, mBLM, recQ helicase homolog
between 1 and 50
2 - 8°C
Detection of mouse BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-BLM antibody A300-570A (lot A300-570A-1) used for IP at 3 µg/mg lysate. BLM was also immunoprecipitated using rabbit anti-BLM antibodies BL2689 and A300-572A at 3 µg/mg lysate. immunoprecipitatesd BLM was blotted using A300-572A at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.