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Rabbit anti-RIF1 Antibody, Affinity Purified - A300-569A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RIF1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-569A
Name:
Rabbit anti-RIF1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RIF1 immobilized on solid support. The epitope recognized by A300-569A maps to a region between residue 2425 and the C-terminus (residue 2472) of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
RAP1 interacting factor homolog, rap1-interacting factor 1 homolog, telomere-associated protein RIF1
Species Reactivity:
Human
Immunogen:
between 2425 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Rif1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody A300-569A (lot A300-569A-5) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Rif1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rif1 antibody A300-569A (lot A300-569A-5) used for IP at 6 µg per reaction. Rif1 was also immunoprecipitated by rabbit anti-Rif1 antibody A300-567A. For blotting immunoprecipitated Rif1 , A300-569A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human RIF1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RIF1 (Cat. No. A300-569A Lot5) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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