Antibody was affinity purified using an epitope specific to MST4/MASK immobilized on solid support. The epitope recognized by A300-566A maps to a region between residue 375 and the C-terminus (residue 416) of human Mammalian Ste20-like Kinase 4 (Mst3 and SOK1-related kinase) using the numbering given in entry NP_057626.2 (GeneID 51765). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
mammalian Ste20-like protein kinase 4, mammalian sterile 20-like 4, MASK, Mst3 and SOK1-related kinase, MST4, MST-4, serine/threonine protein kinase 26, serine/threonine protein kinase MST4, serine/threonine-protein kinase 26, serine/threonine-protein kin
between 375 and C-term
2 - 8°C
Detection of human MST4(MASK) by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB in A; 1 mg for IP, 1/4 of IP loaded in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-MST4(MASK) antibody BL2704 (Cat. No. A300-566A) used at 0.04 µg/ml (A) and 1 µg/ml (B) for WB. MST4(MASK) was immunoprecipitated using rabbit anti-MST4(MASK) antibody BL2703 (Cat. No. A300-565A), which binds an upstream epitope, at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).