Antibody was affinity purified using an epitope specific to MST3/STK24 immobilized on solid support. The epitope recognized by A300-564A maps to a region between residues 325 and 375 of human Mammalian Ste20-like Kinase 3 (Serine/Threonine Kinase 24) using the numbering given in entry NP_003567.2 (GeneID 8428). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory protein Li 95, HEL-S-95, mammalian STE20-like protein kinase 3, MST3, MST-3, MST3B, serine/threonine kinase 24 (STE20 homolog, yeast), serine/threonine-protein kinase 24, STE20, STE20-like kinase 3, STE20-like kinase MST3, sterile 20-
between 325 and 375
2 - 8°C
Detection of human MST3(STK24) by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 IP loaded) and HEK293T (T, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-MST3(STK24) antibody BL2700 (Cat. No. A300-564A) used at 1 µg/ml (B) or 0.04 µg/ml (A) for WB and used at 3 µg/mg lysate for IP (B). MST3(STK24) was also immunoprecipitated using rabbit anti-MST3(STK24) antibody BL2701, which is to a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds.