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Rabbit anti-SYK Antibody, Affinity Purified

Catalogue number:
A300-559A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to SYK immobilized on solid support. The epitope recognized by A300-559A maps to a region between residues 350 and 400 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

p72-Syk, spleen tyrosine kinase, tyrosine-protein kinase SYK

Species Reactivity:

Human

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human SYK by western blot.

Detection of human SYK by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from Ramos cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SYK antibody A300-559A (lot A300-559A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SYK by western blot o

Detection of human SYK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYK antibody A300-559A (lot A300-559A-2) used for IP at 6 µg per reaction. SYK was also immunoprecipitated by a previous lot of this antibody (lot A300-559A-1) and rabbit anti-SYK antibody A300-558A For blotting immunoprecipitated SYK, A300-559A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.