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Rabbit anti-SYK Antibody, Affinity Purified - A300-559A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SYK Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-559A
Name:
Rabbit anti-SYK Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to SYK immobilized on solid support. The epitope recognized by A300-559A maps to a region between residues 350 and 400 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
p72-Syk, spleen tyrosine kinase, tyrosine-protein kinase SYK
Species Reactivity:
Human
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human SYK by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from Ramos cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SYK antibody A300-559A (lot A300-559A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human SYK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SYK antibody A300-559A (lot A300-559A-2) used for IP at 6 µg per reaction. SYK was also immunoprecipitated by a previous lot of this antibody (lot A300-559A-1) and rabbit anti-SYK antibody A300-558A For blotting immunoprecipitated SYK, A300-559A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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