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Rabbit anti-SYK Antibody, Affinity Purified - A300-558A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SYK Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-558A
Name:
Rabbit anti-SYK Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to SYK immobilized on solid support. The epitope recognized by A300-558A maps to a region between residues 250 and 300 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP, IHC

Product Type:
Antibodies
Alternative Names:
p72-Syk, spleen tyrosine kinase, tyrosine-protein kinase SYK
Species Reactivity:
Human
Immunogen:
Between 250 and 300
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human SYK by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, ¼ of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SYK antibody BL2370 (Cat. No. A300-558A) used for IP at 3 µg/mg lysate. SYK was also immunoprecipitated using rabbit anti-SYK antibody BL2371 (Cat. No. A300-559A) at 3 µg/mg lysate. immunoprecipitatesd SYK was blotted using BL2371 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds (B).

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Detection of human SYK by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SYK (Cat. No. A300-558A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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