www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-SYK Antibody, Affinity Purified

Catalogue number:
A300-558A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to SYK immobilized on solid support. The epitope recognized by A300-558A maps to a region between residues 250 and 300 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:

Antibodies

Alternative Names:

p72-Syk, spleen tyrosine kinase, tyrosine-protein kinase SYK

Species Reactivity:

Human

Immunogen:

between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human SYK by immunoprecipit

Detection of human SYK by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, ¼ of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SYK antibody BL2370 (Cat. No. A300-558A) used for IP at 3 µg/mg lysate. SYK was also immunoprecipitated using rabbit anti-SYK antibody BL2371 (Cat. No. A300-559A) at 3 µg/mg lysate. immunoprecipitatesd SYK was blotted using BL2371 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds (B).

Detection of human SYK by immunohistoche

Detection of human SYK by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SYK (Cat. No. A300-558A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB