Antibody was affinity purified using an epitope specific to SYK immobilized on solid support. The epitope recognized by A300-558A maps to a region between residues 250 and 300 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
p72-Syk, spleen tyrosine kinase, tyrosine-protein kinase SYK
between 250 and 300
2 - 8°C
Detection of human SYK by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, ¼ of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SYK antibody BL2370 (Cat. No. A300-558A) used for IP at 3 µg/mg lysate. SYK was also immunoprecipitated using rabbit anti-SYK antibody BL2371 (Cat. No. A300-559A) at 3 µg/mg lysate. immunoprecipitatesd SYK was blotted using BL2371 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds (B).