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Rabbit anti-RPS6 Antibody, Affinity Purified

Catalogue number:
A300-557A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to RPS6 immobilized on solid support. The epitope recognized by A300-557A maps to a region between residue 200 and the C-terminus (residue 249) of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

40S ribosomal protein S6, phosphoprotein NP33, S6, small ribosomal subunit protein eS6

Species Reactivity:

Human, Mouse

Immunogen:

between 200 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse RPS6 by wes

Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody A300-557A (lot A300-557A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RPS6 by western blot

Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody A300-557A (lot A300-557A-2) used for IP at 6 µg per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot A300-557A-1). For blotting immunoprecipitated RPS6, A300-557A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RPS6 by immunohistoch

Detection of human RPS6 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (Cat. No. A300-557A lot 2) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB