Antibody was affinity purified using an epitope specific to RPS6 immobilized on solid support. The epitope recognized by A300-556A maps to a region between residues 100 and 150 of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
40S ribosomal protein S6, phosphoprotein NP33, S6, small ribosomal subunit protein eS6
between 100 and 150
2 - 8°C
Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody A300-556A (lot A300-556A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody A300-556A (lot A300-556A-2) used for IP at 3 µg per reaction. RPS6 was also immunoprecipitated by rabbit anti-RPS6 antibody A300-557A. For blotting immunoprecipitated RPS6, A300-556A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.