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Rabbit anti-RPS6 Antibody, Affinity Purified - A300-556A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RPS6 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-556A
Name:
Rabbit anti-RPS6 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to RPS6 immobilized on solid support. The epitope recognized by A300-556A maps to a region between residues 100 and 150 of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
40S ribosomal protein S6, phosphoprotein NP33, S6, small ribosomal subunit protein eS6
Species Reactivity:
Human, Mouse
Immunogen:
Between 100 and 150
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody A300-556A (lot A300-556A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody A300-556A (lot A300-556A-2) used for IP at 3 µg per reaction. RPS6 was also immunoprecipitated by rabbit anti-RPS6 antibody A300-557A. For blotting immunoprecipitated RPS6, A300-556A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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