Antibody was affinity purified using an epitope specific to GCN2 immobilized on solid support. The epitope recognized by A300-555A maps to a region between residues 1 and 50 of human GCN2 (eIF2AK4, eukaryotic translation initiation factor 2 alpha kinase 4) using the numbering given in entry NP_001013725.2 (GeneID 440275). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, general control nonderepressible 2, PVOD2
between 1 and 50
2 - 8°C
Detection of human GCN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and NIH 3T3 (50 µg) cells. Antibodies: Affinity purified rabbit anti-GCN2 antibody BL2617 (Cat. No. A300-555A) used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). GCN2 was also immunoprecipitated using BL2618, which is an affinity purified antibody to a downstream epitope from GCN2. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).