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Rabbit anti-Raptor Antibody, Affinity Purified - A300-553A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Raptor Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-553A
Name:
Rabbit anti-Raptor Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Raptor immobilized on solid support. The epitope recognized by A300-553A maps to a region between residues 1 and 50 of human Regulatory Associated Protein of mTOR using the numbering given in entry AAM09075.1 (GeneID 57521).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
KOG1, Mip1, p150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, raptor, regulatory-associated protein of mTOR
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Raptor by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody A300-553A (lot A300-553A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody A300-553A (lot A300-553A-3) used for IP at 6 µg per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot A300-553A-2) and rabbit anti-Raptor antibody A300-506A For blotting immunoprecipitated Raptor, A300-553A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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