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Rabbit anti-Raptor Antibody, Affinity Purified

Catalogue number:
A300-553A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Raptor immobilized on solid support. The epitope recognized by A300-553A maps to a region between residues 1 and 50 of human Regulatory Associated Protein of mTOR using the numbering given in entry AAM09075.1 (GeneID 57521). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

KOG1, Mip1, p150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, raptor, regulatory-associated protein of mTOR

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Raptor by western blo

Detection of human Raptor by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Raptor antibody A300-553A (lot A300-553A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Raptor by western blo

Detection of human Raptor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Raptor antibody A300-553A (lot A300-553A-3) used for IP at 6 µg per reaction. Raptor was also immunoprecipitated by a previous lot of this antibody (lot A300-553A-2) and rabbit anti-Raptor antibody A300-506A For blotting immunoprecipitated Raptor, A300-553A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.