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Rabbit anti-MLF1 Antibody, Affinity Purified - A300-552A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MLF1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-552A
Name:
Rabbit anti-MLF1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MLF1 immobilized on solid support. The epitope recognized by A300-552A maps to a region between residue 200 and the C-terminus (residue 256) of human Myeloid Leukemia Factor 1 using the numbering given in entry NP_071888.1 (GeneID 4291).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
myelodysplasia-myeloid leukemia factor 1, myeloid leukemia factor 1, testis tissue sperm-binding protein Li 49e
Species Reactivity:
Human
Immunogen:
Between 200 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MLF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLF1 antibody BL2356 (Cat. No. A300-552A) used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). Human MLF1 was also immunoprecipitated using rabbit anti-MLF1 antibody BL2356, which is to an upstream epitope in MLF1. Detection: Chemiluminescence with an exposure times of 30 seconds (A) and 3 minutes (B).

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