Antibody was affinity purified using an epitope specific to NMI immobilized on solid support. The epitope recognized by A300-551A maps to a region between residue 250 and the C-terminus (residue 307) of human N-myc and STAT interactor using the numbering given in entry NP_004679.1 (GeneID 9111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Nmi, N-myc and STAT interactor, N-myc-interactor
between 250 and C-term
2 - 8°C
Detection of human NMI by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-NMI antibody (Cat. No. A300-551A) used at 0.04 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 30 seconds.