Antibody was affinity purified using an epitope specific to NMI immobilized on solid support. The epitope recognized by A300-550A maps to a region between residues 1 and 50 of human N-myc and STAT interactor using the numbering given in entry NP_004679.1 (GeneID 9111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Nmi, N-myc and STAT interactor, N-myc-interactor
between 1 and 50
2 - 8°C
Detection of human NMI by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NMI antibody A300-550A (lot A300-550A-2) used for IP at 6 µg per reaction. NMI was also immunoprecipitated by rabbit anti-NMI antibody A300-550A-1. For blotting immunoprecipitated NMI, A300-550A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human NMI by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NMI (Cat. No. A300-550A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB