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  3. Rabbit anti-CTCF Antibody, Affinity Purified
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Rabbit anti-CTCF Antibody, Affinity Purified

Catalogue number:
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A300-543A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00
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Estimated delivery Wed 1 May
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

CTCF

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 650 and 700

Alternative Names:

CTCFL paralog; MRD21; CCCTC-binding factor (zinc finger protein); CCCTC-binding factor; 11-zinc finger protein; 11 zinc finger transcriptional repressor; transcriptional repressor CTCF

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Product Description:

Rabbit anti-CTCF Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human CTCF by western blot.
Detection of human CTCF by western blot. Samples: Nuclear extract (50, 15, 5 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse CTCF by western blot.
Detection of mouse CTCF by western blot. Samples: Whole cell lysate (WCL) or Nuclear Extract (NE) (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CTCF by western blot
Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for IP at 6 µg per reaction. CTCF was also immunoprecipitated by rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041). For blotting immunoprecipitated CTCF, A700-041 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Detection of human CTCF by immunohistoch
Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
Detection of mouse CTCF by immunohistoch
Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
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