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Rabbit anti-CTCF Antibody, Affinity Purified

Catalogue number:
A300-543A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to CTCF immobilized on solid support. The epitope recognized by A300-543A maps to a region between residues 650 and 700 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF

Species Reactivity:

Human, Mouse

Immunogen:

Between 650 and 700

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human CTCF by western blot.

Detection of human CTCF by western blot. Samples: Nuclear extract (50, 15, 5 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse CTCF by western blot.

Detection of mouse CTCF by western blot. Samples: Whole cell lysate (WCL) or Nuclear Extract (NE) (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human CTCF by western blot

Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for IP at 6 µg per reaction. CTCF was also immunoprecipitated by rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041). For blotting immunoprecipitated CTCF, A700-041 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

Detection of human CTCF by immunohistoch

Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

Detection of mouse CTCF by immunohistoch

Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).