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Rabbit anti-CTCF Antibody, Affinity Purified - A300-543A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-CTCF Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-543A
Name:
Rabbit anti-CTCF Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to CTCF immobilized on solid support. The epitope recognized by A300-543A maps to a region between residues 650 and 700 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF
Species Reactivity:
Human, Mouse
Immunogen:
Between 650 and 700
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human CTCF by western blot. Samples: Nuclear extract (50, 15, 5 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse CTCF by western blot. Samples: Whole cell lysate (WCL) or Nuclear Extract (NE) (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-5) used for IP at 6 µg per reaction. CTCF was also immunoprecipitated by rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (A700-041). For blotting immunoprecipitated CTCF, A700-041 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

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Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

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Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (A300-543A lot 5) used at 1:2000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

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