Antibody was affinity purified using an epitope specific to REST immobilized on solid support. The epitope recognized by A300-540A maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DFNA27, GINGF5, HGF5, neural-restrictive silencer factor, neuron restrictive silencer factor, NRSF, RE1-silencing transcription factor, repressor binding to the X2 box, WT6, X2 box repressor, XBR
between 1050 and C-term
2 - 8°C
Detection of human REST by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody A300-540A (lot A300-540A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human REST by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- REST (Cat. No. A300-540A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).