Antibody was affinity purified using an epitope specific to REST immobilized on solid support. The epitope recognized by A300-539A maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DFNA27, GINGF5, HGF5, neural-restrictive silencer factor, neuron restrictive silencer factor, NRSF, RE1-silencing transcription factor, repressor binding to the X2 box, WT6, X2 box repressor, XBR
between 800 and 850
2 - 8°C
Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody A300-539A (lot A300-539A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated REST, A300-539A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.