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Rabbit anti-REST Antibody, Affinity Purified

Catalogue number:
100 µg
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Antibody was affinity purified using an epitope specific to REST immobilized on solid support. The epitope recognized by A300-539A maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:


Alternative Names:

DFNA27, GINGF5, HGF5, neural-restrictive silencer factor, neuron restrictive silencer factor, NRSF, RE1-silencing transcription factor, repressor binding to the X2 box, WT6, X2 box repressor, XBR

Species Reactivity:



between 800 and 850

Storage Temp:

2 - 8°C

Antibody Host:


Antibody Isotype:

Whole IgG

Antibody Type:




Detection of human REST by western blot

Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody A300-539A (lot A300-539A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated REST, A300-539A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human REST by immunohistoch

Detection of human REST by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REST (Cat. No. A300-539A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB