Antibody was affinity purified using an epitope specific to Pds5B immobilized on solid support. The epitope recognized by A300-538A maps to a region between residue 1400 and the C-terminus (residue 1447) of human Precocious Dissociation of Sisters-5B using the numbering given in entry CAB69911.1 (GeneID 23047). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
androgen induced inhibitor of proliferation, androgen-induced proliferation inhibitor, androgen-induced prostate proliferative shutoff-associated protein AS3, androgen-induced shutoff 3, APRIN, AS3, CG008, sister chromatid cohesion protein PDS5 homolog B
between 1400 and C-term
2 - 8°C
Detection of human and mouse Pds5b by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Pds5b antibody A300-538A (lot A300-538A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Pds5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pds5b antibody A300-538A (lot A300-538A-2) used for IP at 3 µg per reaction. Pds5b was also immunoprecipitated by rabbit anti-Pds5b antibody A300-537A. For blotting immunoprecipitated Pds5b, A300-538A was used at 1 µg/ml. Chemiluminescence with an exposure time of 75 seconds.
Detection of human Pds5B by immunohistochemistry. Sample: FFPE section of human lung small cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B (Cat. No. A300-538A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB