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Rabbit anti-Phospho BID (S78) Antibody, Affinity Purified - A300-528A - 50 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Phospho BID (S78) Antibody, Affinity Purified
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Size: 50 µg
£322.00
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Catalogue number:
A300-528A
Name:
Rabbit anti-Phospho BID (S78) Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BID phosphorylated on Serine 78 immobilized on solid support. The epitope recognized by A300-528A maps to phosphorylated Serine 78 and surrounding residues of mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB

Product Type:
Antibodies
Alternative Names:
2700049M22Rik, AI875481, AU022477, BH3-interacting domain death agonist, BID, p22 BID
Species Reactivity:
Mouse
Immunogen:
Surrounding serine 78
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of mouse Phospho-BID (S78) after Treatment with Etoposide. Samples: Whole cell lysate (50 and 80 µg for WB; 1 mg for IP, 20% of IP loaded) from NIH 3T3 cells treated with 100 µM etoposide for 3 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-phospho BID (S78) antibody A300-528A (lot A300-528A-2) used for WB at 1 µg/ml (upper panels of A and B). Rabbit anti-BID antibody BL1079 was used for IP of BID and phospho-BID and for detection of total BID (lower panels of A and B). Detection: Chemiluminescence with exposure times of 3 minutes (upper panels of A and B).

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