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Rabbit anti-DHX36 Antibody, Affinity Purified

Catalogue number:
A300-525A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to DHX36 immobilized on solid support. The epitope recognized by A300-525A maps to a region between residue 950 and the C-terminus (residue 1008) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 36 using the numbering given in entry NP_065916.1 (GeneID 170506). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

ATP-dependent RNA helicase DHX36, DDX36, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 36, DEAD/H box polypeptide 36, DEAH (Asp-Glu-Ala-His) box polypeptide 36, DEAH box protein 36, DEAH-box protein 36, G4 resolvase-1, G4R1, G4-resolvase-1, MLEL1, MLE-like

Species Reactivity:

Human, Mouse

Immunogen:

between 950 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DHX36 by western blot

Detection of human DHX36 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (50µg) and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody A300-525A (lot A300-525A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse DHX36 by western blot

Detection of mouse DHX36 by western blot. Samples: Whole cell lysate from NIH 3T3 (50µg), TCKM (50µg) and Renca (50µg) cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody A300-525A (lot A300-525A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DHX36 by western blot

Detection of human DHX36 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX36 antibody A300-525A (lot A300-525A-2) used for IP at 6 µg/mg lysate. DHX36 was also immunoprecipitated by a previous lot (A300-525A-1) of this antibody. For blotting immunoprecipitated DHX36, A300-525A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human DHX36 by immunohistoc

Detection of human DHX36 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-DHX36 (Cat. No. A300-525A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB