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Bromodomain-Containing (BRD) Antibodies
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Rabbit anti-DDX1 Antibody, Affinity Purified - A300-521A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DDX1 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-521A
Name:
Rabbit anti-DDX1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DDX1 immobilized on solid support. The epitope recognized by A300-521A maps to a region between residue 650 and the C-terminus (residue 697) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase DDX1, DBP-RB, DEAD (Asp-Glu-Ala-Asp) box helicase 1, DEAD (Asp-Glu-Ala-Asp) box polypeptide 1, DEAD box polypeptide 1, DEAD box protein 1, DEAD box protein retinoblastoma, DEAD box-1, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
Species Reactivity:
Human, Mouse
Immunogen:
Between 650 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse DDX1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX1 antibody A300-521A (lot A300-521A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human DDX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX1 antibody A300-521A (lot A300-521A-2) used for IP at 6 µg per reaction. DDX1 was also immunoprecipitated by a previous lot of this antibody (lot A300-521A-1). For blotting immunoprecipitated DDX1, A300-521A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human DDX1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX1 (Cat. No. A300-521A Lot2) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB

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Detection of mouse DDX1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-DDX1 (Cat. No. A300-521A Lot2) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB

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