Antibody was affinity purified using an epitope specific to DNA-PKcs immobilized on solid support. The epitope recognized by A300-518A maps to a region between residues 2100 and 2150 of human DNA-Dependent Protein Kinase, catalytic subunit using the numbering given in entry NP_008835.5 (GeneID 5591). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA-dependent protein kinase catalytic subunit, DNAPK, DNA-PK catalytic subunit, DNAPKc, DNA-PKC, DNA-PKcs, DNPK1, hyper-radiosensitivity of murine scid mutation, complementing 1, HYRC, HYRC1, IMD26, p350, p460, protein kinase, DNA-activated, catalytic po
between 2100 and 2150
2 - 8°C
Detection of human DNA-PKcs by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA-PKcs antibody BL2404 (Cat. No. A300-518A) used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies BL2402 (Cat. No. A300-516A), BL2403 (Cat. No. A300-517A) and BL2406 (Cat. No. A300-519A). Detection: Chemiluminescence with an exposure time of 3 minutes.