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Rabbit anti-DNA-PKcs Antibody, Affinity Purified

Catalogue number:
A300-518A
Size:
20 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to DNA-PKcs immobilized on solid support. The epitope recognized by A300-518A maps to a region between residues 2100 and 2150 of human DNA-Dependent Protein Kinase, catalytic subunit using the numbering given in entry NP_008835.5 (GeneID 5591). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

DNA-dependent protein kinase catalytic subunit, DNAPK, DNA-PK catalytic subunit, DNAPKc, DNA-PKC, DNA-PKcs, DNPK1, hyper-radiosensitivity of murine scid mutation, complementing 1, HYRC, HYRC1, IMD26, p350, p460, protein kinase, DNA-activated, catalytic po

Species Reactivity:

Human

Immunogen:

between 2100 and 2150

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DNA-PKcs by western b

Detection of human DNA-PKcs by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DNA-PKcs antibody BL2404 (Cat. No. A300-518A) used at 0.1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). DNA-PKcs was also immunoprecipitated using rabbit anti-DNA-PKcs antibodies BL2402 (Cat. No. A300-516A), BL2403 (Cat. No. A300-517A) and BL2406 (Cat. No. A300-519A). Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DNA-PKcs by immunohis

Detection of human DNA-PKcs by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DNA-PKcs (Cat. No. A300-518A) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB