Antibody was affinity purified using an epitope specific to Roquin immobilized on solid support. The epitope recognized by A300-515A maps to a region between residue 1075 and the C-terminus (residue 1133) of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
probable E3 ubiquitin-protein ligase Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198, RNF198, ROQUIN, roquin-1
between 1075 and C-term
2 - 8°C
Detection of human Roquin by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T, 50 µg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody BL2416 (Cat. No. A300-515A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody BL2413 (Cat. No. A300-514A) at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.