Rabbit anti-Roquin Antibody, Affinity Purified - A300-515A - 20 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Roquin immobilized on solid support. The epitope recognized by A300-515A maps to a region between residue 1075 and the C-terminus (residue 1133) of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human Roquin by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T, 50 µg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody BL2416 (Cat. No. A300-515A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody BL2413 (Cat. No. A300-514A) at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
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