Antibody was affinity purified using an epitope specific to RanBP1 immobilized on solid support. The epitope recognized by A300-512A maps to a region between residues 150 and the C-terminus (residue 201) of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HpaII tiny fragments locus 9A, HTF9A, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein, testis secretory sperm-binding protein Li 221n
between 150 and C-term
2 - 8°C
Detection of human RanBP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody A300-512A (lot A300-512A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.