Antibody was affinity purified using an epitope specific to hnRNP-H immobilized on solid support. The epitope recognized by A300-511A maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein, heterogeneous nuclear ribonucleoprotein H, heterogeneous nuclear ribonucleoprotein H1 (H), hnRNP H, hnRNPH, HNRPH, HNRPH1
between 400 and C-term
2 - 8°C
Detection of human and mouse hnRNP H by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP H antibody A300-511A (lot A300-511A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. hnRNP H was also immunoprecipitated by a prior lot (lot A300-511A-1) of this antibody. Detection: Chemiluminescence with exposure times 30 second (A and B).