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Rabbit anti-hnRNP-H Antibody, Affinity Purified - A300-511A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-hnRNP-H Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-511A
Name:
Rabbit anti-hnRNP-H Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to hnRNP-H immobilized on solid support. The epitope recognized by A300-511A maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
epididymis secretory sperm binding protein, heterogeneous nuclear ribonucleoprotein H, heterogeneous nuclear ribonucleoprotein H1 (H), hnRNP H, hnRNPH, HNRPH, HNRPH1
Species Reactivity:
Human, Mouse
Immunogen:
Between 400 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse hnRNP H by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP H antibody A300-511A (lot A300-511A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. hnRNP H was also immunoprecipitated by a prior lot (lot A300-511A-1) of this antibody. Detection: Chemiluminescence with exposure times 30 second (A and B).

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Detection of human hnRNP H by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H (Cat. No. A300-511A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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