Rabbit anti-DDX17 Antibody, Affinity Purified - A300-509A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to DDX17 immobilized on solid support. The epitope recognized by A300-509A maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IHC
Detection of human DDX17 by western blot. Samples: Whole cell lysate (15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody A300-509A (lot A300-509A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human DDX17 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 (Cat. No. A300-509A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of mouse and rat DDX17 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody A300-509A (lot A300-509A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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