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Rabbit anti-AMPK alpha 1 Antibody, Affinity Purified - A300-507A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-AMPK alpha 1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-507A
Name:
Rabbit anti-AMPK alpha 1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to AMPK alpha 1 immobilized on solid support. The epitope recognized by A300-507A maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, acetyl-CoA carboxylase kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK,
Species Reactivity:
Human, Mouse
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse AMPK alpha 1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-AMPK alpha 1 antibody A300-507A (lot A300-507A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human AMPK alpha 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-AMPK alpha 1 antibody A300-507A (lot A300-507A-3) used for IP at 3 µg per reaction. AMPK alpha 1 was also immunoprecipitated by a previous lot of this antibody (lot A300-507A-2). For blotting immunoprecipitated AMPK alpha 1, A300-507A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human smooth muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 (Cat. No. A300-507A Lot 3) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB

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