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Rabbit anti-mTOR Antibody, Affinity Purified

Catalogue number:
A300-504A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to mTOR immobilized on solid support. The epitope recognized by A300-504A maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FRAP,

Species Reactivity:

Human

Immunogen:

between 200 and 250

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human mTOR by western blot.

Detection of human mTOR by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody A300-504A (lot A300-504A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human mTOR by western blot

Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody A300-504A (lot A300-504A-2) used for IP at 6 µg per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody A301-143A. For blotting immunoprecipitated mTOR, A300-504A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.