Antibody was affinity purified using an epitope specific to mTOR immobilized on solid support. The epitope recognized by A300-503A maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FRAP,
between 200 and 250
2 - 8°C
Detection of human mTOR by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody A300-503A (lot A300-503A-7) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody A300-503A (lot A300-503A-7) used for IP at 3 µg per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody A300-504A. For blotting immunoprecipitated mTOR, A300-503A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.