Antibody was affinity purified using an epitope specific to CamK4 immobilized on solid support. The epitope recognized by A300-494A maps to a region between residue 425 and the C-terminus (residue 473) of human Calcium/Calmodulin-dependent Protein Kinase IV using the numbering given in Swiss-Prot entry Q16566 (GeneID 814). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
brain Ca(2+)-calmodulin-dependent protein kinase type IV, brain Ca++-calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV catalytic chain, CAM kinase- GR, CAM
between 425 and C-term
2 - 8°C
Detection of human CamK4 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from Jurkat (5, 15 and 50 µg) and HeLa (50 µg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CamK4 antibody BL1836 (Cat. No. A300-494A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). CamK4 was also immunoprecipitated using CamK4 antibodies BL1834 (Cat. No. A300-492A) and BL1835 (Cat. No. A300-493A) used at 3 µg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 30 seconds.